Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles
نویسندگان
چکیده
منابع مشابه
Myosin heavy chain isoforms in human extraocular muscles.
PURPOSE To investigate the myosin heavy chain (MyHC) composition of human extraocular (EOM) and levator palpebrae (LP) muscle fibers. METHODS Adult human EOMs and LP were studied with SDS-PAGE, immunoblots, and immunocytochemistry, with antibodies against six MyHC isoforms. Myofibrillar adenosine triphosphatase (mATPase) and reduced nicotinamide adenine dinucleotide (NADH)-TR activity and fib...
متن کاملFunctional characterization of vertebrate nonmuscle myosin IIB isoforms using Dictyostelium chimeric myosin II.
The alternatively spliced isoform of nonmuscle myosin II heavy chain B (MHC-IIB) with an insert of 21 amino acids in the actin-binding surface loop (loop 2), MHC-IIB(B2), is expressed specifically in the central nervous system of vertebrates. To examine the role of the B2 insert in the motor activity of the myosin II molecule, we expressed chimeric myosin heavy chain molecules using the Dictyos...
متن کاملPostnatal changes in the developing rat extraocular muscles.
PURPOSE To examine the distribution and timing of expression of nonmuscle myosin IIB (nmMyH IIB) and the extraocular muscle (EOM)-specific myosin (EO-MyHC) during postnatal development of the rat extraocular muscles. METHODS Whole orbits were collected from postnatal development day (P) 1 through P30 from Sprague-Dawley rats. Samples were analyzed by immunofluorescence microscopy and Western ...
متن کاملTwo regions of the tail are necessary for the isoform-specific functions of nonmuscle myosin IIB.
To function in the cell, nonmuscle myosin II molecules assemble into filaments through their C-terminal tails. Because myosin II isoforms most likely assemble into homo-filaments in vivo, it seems that some self-recognition mechanisms of individual myosin II isoforms should exist. Exogenous expression of myosin IIB rod fragment is thus expected to prevent the function of myosin IIB specifically...
متن کاملE03-06-0359 Revised Nonmuscle Myosin IIB Is Involved in the Guidance of Fibroblast Migration
Running Title: Role of Myosin IIB in Fibroblast Migration
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ژورنال
عنوان ژورنال: Experimental Cell Research
سال: 2010
ISSN: 0014-4827
DOI: 10.1016/j.yexcr.2010.03.018